MuViSPIM and the InViSPIM

Workshop flyer

Both systems we will have available at the workshop are dedicated to live imaging: They are equipped with water-dipping lenses.

The MuVi SPIM and the InVi SPIM both have 6 Laser lines, covering the spectrum from 445nm to 685nm.

MuVi: 445nm, 488nm, 515nm, 561nm, 594nm, and 642nm

InVi: 488, 515, 561 and 642 nm

On the MuVi-SPIM, samples are embedded in low melting point agarose and supported from below, which gives them good stability. If there is need, we can also use FEP tubes. Typical samples are e.g. zebrafish / drosophila embryos, or thelike – everything that benefits from the twofold symmetry and the possibility of sample rotation: The MuVi SPIM is equipped with two illumination and two detection arms. Multi-view acquisition is possible (incl. sample rotation) such that an isotropic 3D resolution can be achieved.
The MuVi-SPIM is equipped with a photomanipulation module that enables ablation (manipulation) in (of) the sample.

The InVi-SPIM has an inverted light sheet geometry, dedicated to small and delicate samples such as cells, spheroids, organoids and embryos.
It features a mounting approach by means of gravity into a “trough” of FEP foil through which the specimen is illuminated and imaged from below.  The specimen can freely develop, as there is no physical restriction, no gel, no embedding.
The InVi-SPIM allows medium throughput imaging, and we offer full atmosphere control (Temperature, CO₂, O₂, N₂) [TBC with Clara].

In the workshop we introduce you to the MuVi-SPIM / InVi-SPIM system, and guide you through the necessary steps to run high-speed light sheet microscopy. You will learn how to mount samples, set up an experiment, acquire a time-lapse movie, process and visualize the acquired data.

To organize the different slots, we put up a doodle, where you can register:

MuVi: https://doodle.com/poll/6d67cyktpskbqcsy

InVi: https://doodle.com/poll/sty5rkde4236ka88

(Max. 5 participants per slot)