The W.M. Keck Center for Cellular Imaging (KCCI), a university imaging center at the University of Virginia, is sponsoring an advanced practical course on Förster (fluorescence) resonance energy transfer
(FRET) and fluorescence lifetime imaging (FLIM) microscopy.

Attendees are expected to be familiar with
the basics of fluorescence microscopy. The curriculum, after a brief introduction to the principles of fluorescence, microscopy, fluorohopres, FRET and FLIM, will concentrate on the practical aspects, hands-on individual instruction at the instruments followed by data analysis and interpretation.

Lectures and after dinner problem-solving discussions will address questions of fluortophore choices, the most suitable systems to achieve specific research objectives, qualitative vs. quantitative analysis and many more related subjects. Participants will also be introduced to a unique image processing and analysis software (PFRET).

10+ different and advanced microscopy systems will be available for a maximum of 25 students. With 10 internationally recognized faculty in attendance, there is ample opportunity to interact with experts
formally or informally. Live-cell specimens are provided. Participant’s own specimens are welcome.