We would like to offer a mini hands-on workshop on CARS microscopy for PhD students and SDU staff. The course is one day course and includes theory introduction (2 hours) and lab exercises (4 hours).

Workshop corresponds to 0.5 ECTS.

Max. number of participants: 4.

Date: 31.08.2017, Registration deadline 10.08.2017

For non-SDU participants price: 1000 DKK

Responsible teacher: Jonathan R. Brewer (brewer(at)bmb.sdu.dk).

Contact Vita Solovyeva for the enrollment: vita(at)sdu.dk

CARS is a label-free imaging technique that is capable of real-time, non-perturbative examination of living cells and organisms based on molecular vibrational spectroscopy.

The advantages of CARS are:

1. Label-free method.
2. More sensitive than spontaneous Raman microscopy.
3. Easily detected in the presence of one-photon fluorescence.
4. Penetrate to depths of nearly 0.4 mm, allowing imaging in thick tissues.
5. Low photodamage.

Applications:

• Lipid imaging
• Mapping out protein distributions in tissues and cells
• Diffusion of water and drugs
• Visualization of chemical composition of polymer films

Reference: Annu. Rev. Anal. Chem. 2008. 1:883–909