The Zeiss LS7 is a flexible Gaussian-beam light sheet (LS) or single plane illumination microscopy (SPIM), capable of optimally imaging small and large samples (from organoids to full embryos), both live (over time) or fixed (cleared) samples at great depths, thanks to its wide objective selection. Light sheet technologies enable for fast, gentle and deep imaging of large and/or sensitive samples while providing excellent optical sectioning due to the nature of single plane illumination.

The system boasts two illumination objectives (5x and 10x) that create a two-sided Gaussian light sheet, and a single detection objective (to choose from 5x, 10x or 20x) placed perpendicularly to the illumination plane. Signal detection is achieved by means of two Edge 4.2 sCMOS cameras by PCO. The lasers provided for excitation are 405, 488, 561 and 638 nm. This enables for collection of large sample volumes from multiple individual focal planes at various levels of detail, from mesoscopic imaging to single cell resolution.

 

More info can be found here – LS7 Light Sheet – University of Copenhagen (ku.dk)