High content imaging (HCI) involves automated collection and analysis of images to provide quantitative data on a range of functional and morphological changes in cells, spheroids or organoids. It has become a widely used tool in cell biology and drug discovery, for example in screening of drug candidates, fragments, RNAi, miR and phenotypic screens using CRISPR-Cas9, amongst other. This meeting will introduce the power of HCI to new or inexperienced users, but will also cover current HCI case studies from leaders in the field and advances in HCI techniques and analysis. Major suppliers of HCI software and analysis will be present to demonstrate applications and uses of their HCI equipment and software. Anyone interested in HCI, whether starting out in the field or an experienced user, will benefit from attending