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Electron Microscopy Course

October 15 - October 26

(Deadline: September 10)

Aim and content

This course is free of charge for PhD students at Danish universities (except Copenhagen Business School). All other participants must pay the course fee.
Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list.

Content
This course is suitable not only for beginners in electron microscopy, but also for those who already use the electron microscope in their work and now want to extend their knowledge of basic principles and more specialised techniques. The course is run in collaboration with the Royal Microscopical Society (RMS).

Week 1 provides an essential foundation in the basic principles of electron microscopy, focusing on room temperature techniques which are the basis of ultra-structural studies of cellular systems, covering topics such as principles of transmission and scanning electron microscopy, electron sources, vacuum systems, specimen-electron interactions and diffraction, electron optics, and electromagnetic lenses. Biological specimen preparation will constitute a major part of the course, including methods of chemical fixation.

Week 2 provides a comprehensive introduction to cryo EM, an approach which includes structural determination of macromolecules and macromolecular machinery by electron microscopy which in 2017 attracted the Nobel prize in chemistry. The course will cover the technology, practical application and principles of cryo EM, including cryo-preservation by high pressure freezing and single particle analysis. Advanced electron microscope techniques such as immunogold labeling, electron tomography, and data analysis/visualization of molecular structure data will be introduced towards the end of the course.

The state-of-the-art facilities available at CFIM (www.cfim.ku.dk) including a Titan Krios allow for a strong practical element, with time for each student to gain hands-on experience of both transmission and scanning electron microscopes. The course will be run by experienced microscopists in a relaxed atmosphere with the aim of promoting discussion and exchange of ideas between students and tutors.

Participants
PhD students

Language
English

Form
Lectures,exercises

Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:

Medical and Molecular Imaging
Neuroscience
Cellular and Genetic Medicine

Course director
Professor Klaus Qvortrup,

Teachers
Roland Fleck, Kings College London; Pippa Hawes, The Pirbright Institute; Kenton Arkill, University of Nottingham; Peter Nellist, Oniversity of Oxford; Klaus Qvortrup, University of Copenhagen; Zhila Nikrozi, University of Copenhagen; Cristiano di Benedetto, University of Copenhagen; Michael Johnson, University of Copenhagen; Tillmann Hans Pape, University of Copenhagen; Pablo Mesa, University of Copenhagen; Gema Vizcay, Kings College London; Leanne Glover, Kings College London; Company representatives from Thermo Fisher (formerly FEI) and Leica Microsystems.

Course secretary
Ida Marie Bergman Rasmussen (idambr@sund.ku.dk)

Dates
15.10 – 26.10 2018.

Course location
The Panum Institute

Registration: Please register before 10 September 2018

Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrollment.

 

Note: All applicants are asked to submit invoice details in case of no-show, late cancellation or obligation to pay the course fee (typically non-PhD students). If you are a PhD student, your participation in the course must be in agreement with your principal supervisor.

Details

Start:
October 15
End:
October 26
Event Category:
Website:
https://phdcourses.ku.dk/DetailKursus.aspx?id=105246&sitepath=SUND
Deadline
September 10

Organizer

Klaus Qvortrup
Email:
idambr@sund.ku.dk

Venue

Panum Institute
Blegdamsvej 3B, Copenhagen + Google Map