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Electron Microscopy Course
October 15, 2018 - October 26, 2018(Deadline: September 10, 2018)
Aim and content
Anyone can apply for the course, but if you are not a PhD student, you will be placed on the waiting list for the course until enrollment deadline. After the deadline of enrollment, available seats will be allocated to students on the waiting list.
This course is suitable not only for beginners in electron microscopy, but also for those who already use the electron microscope in their work and now want to extend their knowledge of basic principles and more specialised techniques. The course is run in collaboration with the Royal Microscopical Society (RMS).
Week 1 provides an essential foundation in the basic principles of electron microscopy, focusing on room temperature techniques which are the basis of ultra-structural studies of cellular systems, covering topics such as principles of transmission and scanning electron microscopy, electron sources, vacuum systems, specimen-electron interactions and diffraction, electron optics, and electromagnetic lenses. Biological specimen preparation will constitute a major part of the course, including methods of chemical fixation.
Week 2 provides a comprehensive introduction to cryo EM, an approach which includes structural determination of macromolecules and macromolecular machinery by electron microscopy which in 2017 attracted the Nobel prize in chemistry. The course will cover the technology, practical application and principles of cryo EM, including cryo-preservation by high pressure freezing and single particle analysis. Advanced electron microscope techniques such as immunogold labeling, electron tomography, and data analysis/visualization of molecular structure data will be introduced towards the end of the course.
The state-of-the-art facilities available at CFIM (www.cfim.ku.dk) including a Titan Krios allow for a strong practical element, with time for each student to gain hands-on experience of both transmission and scanning electron microscopes. The course will be run by experienced microscopists in a relaxed atmosphere with the aim of promoting discussion and exchange of ideas between students and tutors.
Relevance to graduate programmes
The course is relevant to PhD students from the following graduate programmes at the Graduate School of Health and Medical Sciences, UCPH:
Medical and Molecular Imaging
Cellular and Genetic Medicine
Professor Klaus Qvortrup,
Roland Fleck, Kings College London; Pippa Hawes, The Pirbright Institute; Kenton Arkill, University of Nottingham; Peter Nellist, Oniversity of Oxford; Klaus Qvortrup, University of Copenhagen; Zhila Nikrozi, University of Copenhagen; Cristiano di Benedetto, University of Copenhagen; Michael Johnson, University of Copenhagen; Tillmann Hans Pape, University of Copenhagen; Pablo Mesa, University of Copenhagen; Gema Vizcay, Kings College London; Leanne Glover, Kings College London; Company representatives from Thermo Fisher (formerly FEI) and Leica Microsystems.
Ida Marie Bergman Rasmussen (firstname.lastname@example.org)
15.10 – 26.10 2018.
The Panum Institute
Registration: Please register before 10 September 2018
Admission to PhD students from Danish universities will be allocated on a first-come, first-served basis and according to the rules in force.
Applications from other participants will be considered after the last day of enrollment.