The objective of this theoretical and practical course is to introduce advanced microscopy techniques such as high-speed live cell imaging, fluorescence lifetime imaging and super resolution. The course includes the Danish BioImaging Network’s scientific symposium, a conference that allows the students to network with the Danish microscopist community. The course comprises theory and show cases benefitting from invited international experts as well as CAB’s advanced instrumentation. The course participants are awarded 5 ECTS points.