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PRODID:-//Danish BioImaging Network - ECPv4.9.1.1//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-ORIGINAL-URL:https://www.danishbioimaging.dk
X-WR-CALDESC:Events for Danish BioImaging Network
BEGIN:VEVENT
DTSTART;VALUE=DATE:20180220
DTEND;VALUE=DATE:20180223
DTSTAMP:20260716T152843
CREATED:20180209T125716Z
LAST-MODIFIED:20180216T104833Z
UID:1863-1519084800-1519343999@www.danishbioimaging.dk
SUMMARY:LIGHT SHEET MICROSCOPY WORKSHOP in Copenhagen (3 hours slots)
DESCRIPTION:MuViSPIM and the InViSPIM \nWorkshop flyer \nBoth systems we will have available at the workshop are dedicated to live imaging: They are equipped with water-dipping lenses. \nThe MuVi SPIM and the InVi SPIM both have 6 Laser lines\, covering the spectrum from 445nm to 685nm. \nMuVi: 445nm\, 488nm\, 515nm\, 561nm\, 594nm\, and 642nm \nInVi: 488\, 515\, 561 and 642 nm \n  \nOn the MuVi-SPIM\, samples are embedded in low melting point agarose and supported from below\, which gives them good stability. If there is need\, we can also use FEP tubes. Typical samples are e.g. zebrafish / drosophila embryos\, or thelike – everything that benefits from the twofold symmetry and the possibility of sample rotation: The MuVi SPIM is equipped with two illumination and two detection arms. Multi-view acquisition is possible (incl. sample rotation) such that an isotropic 3D resolution can be achieved.\nThe MuVi-SPIM is equipped with a photomanipulation module that enables ablation (manipulation) in (of) the sample. \n  \nThe InVi-SPIM has an inverted light sheet geometry\, dedicated to small and delicate samples such as cells\, spheroids\, organoids and embryos.\nIt features a mounting approach by means of gravity into a “trough” of FEP foil through which the specimen is illuminated and imaged from below.  The specimen can freely develop\, as there is no physical restriction\, no gel\, no embedding.\nThe InVi-SPIM allows medium throughput imaging\, and we offer full atmosphere control (Temperature\, CO2\, O2\, N2) [TBC with Clara]. \n  \n  \nIn the workshop we introduce you to the MuVi-SPIM / InVi-SPIM system\, and guide you through the necessary steps to run high-speed light sheet microscopy. You will learn how to mount samples\, set up an experiment\, acquire a time-lapse movie\, process and visualize the acquired data. \n  \nTo organize the different slots\, we put up a doodle\, where you can register: \n  \nMuVi: https://doodle.com/poll/6d67cyktpskbqcsy \n  \nInVi: https://doodle.com/poll/sty5rkde4236ka88 \n  \n(Max. 5 participants per slot) \n  \n  \n
URL:https://www.danishbioimaging.dk/event/light-sheet-microscopy-workshop-in-copenhagen-3-hours-slots/
LOCATION:KU\, Panum Institute\, building 15. Blegdamsvej 3B
CATEGORIES:Workshops
ORGANIZER;CN="Juergen%20Mayer%2C%20Bruker%20Nano%20Surfaces%20Division":MAILTO:Juergen.Mayer@bruker.com
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