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VERSION:2.0
PRODID:-//Danish BioImaging Network - ECPv4.9.1.1//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-ORIGINAL-URL:https://www.danishbioimaging.dk
X-WR-CALDESC:Events for Danish BioImaging Network
BEGIN:VEVENT
DTSTART;VALUE=DATE:20190729
DTEND;VALUE=DATE:20190803
DTSTAMP:20260716T114912
CREATED:20181205T211606Z
LAST-MODIFIED:20181205T211606Z
UID:2935-1564358400-1564790399@www.danishbioimaging.dk
SUMMARY:Hands-on Flow Cytometry - Learning by Doing!
DESCRIPTION:Course Overview\nFlow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. Utilising Flow Cytometry we can identify and quantify many different parameters from heterogeneous samples\, analyse the corresponding state of proliferation or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2017\, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum. \nAudience\nThe course is designed for researchers at any step of their career and will cover the subject from basic principles to complex assays. Prior basic working knowledge of Flow Cytometry is of advantage\, but not strictly a must. \nModules/Resources\n\nIn-depth principles of Flow Cytometry\nQuality assessment and instrument validation\nPrinciples and guidelines for experimental design\, especially for multi-color Flow Cytometry\nStaining of tissues with cell surface antigens\nStaining cell cycle profiles in fixed and live cells\nAssessing proliferation and cell division\nThe beauty and burdens of fluorescence proteins in Flow Cytometry\nDetailed multi-sessions on analysing data from Flow Cytometers in cooperation with FlowJo\nIf requested\, exploration of very-high parametric Flow Cytometry data highlighting important experimental design and analysis issues with real data from collaborating laboratories.\n\nLearning Outcomes\nOur goal for this course is to teach the basic of this technology with a very big emphasis on practical work at the instruments. Nothing is better than learning by doing. We have a great set of experienced trainers in this technology delivering lectures and accompanying you into the lab doing flow on your own. We will have support from FlowJo delivering the basics and some advanced features in multiple sessions. This in-depth five-day course will push your knowledge and skills preparing you for any flow projects ahead on your research path. \nAcknowledgements\nThis course will happen with the generous support from Becton Dickinson and ThermoFisher providing access to Flow Cytometers\, reagents\, and whole assay units. We are very grateful to BD Treestar providing training support and access to FlowJo for the duration of the course. \n\n  \n\n
URL:https://www.danishbioimaging.dk/event/hands-on-flow-cytometry-learning-by-doing/
LOCATION:Heidelberg\, Germany
CATEGORIES:Courses
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