C267/02 Introduction to Fluorescence Microscopy

ECTS: 6.00
GP: ClinFO
Responsible: Lene Niemann Nejsum
Secretary: Annette Madsen
Application deadline: 23-7-2018

Title: Introduction to Fluorescence Microscopy

Reg.no.: C267/02

is being offered by the Graduate School of Health, Aarhus University, fall 2018.

Criteria for participation: University degree in medicine, dentistry, nursing, or Master’s degree in other fields and/or postgraduate research fellows (PhD students and research-year medical students).

Aim: The aim is to give the participants an overview of basic fluorescence microscopy techniques, sample preparation, image acquisition and imaging analysis.

Learning outcomes: The students should obtain a basic knowledge of different fluorescence microscopy techniques, sample preparation, image acquisition and image analysis. This should enable them to prepare their samples for fluorescence microscopy, choose the appropriate microscope setup for their research purpose, acquire images and subsequently perform quantitative analyses to generate publication figures, thus enabling them to participate in research projects involving fluorescence microscopy.

Content: 1. Overview of basic fluorescence microscopy methods including white light microscopy, wide-field fluorescence microscopy and confocal microscopy as well as optics, cameras and photodetectors. 2. Introduction to sample preparation (hands on) of cells and paraffin embedded tissue sections for fluorescence microscopy. 3. Introduction to image analysis using ImageJ Fiji; this includes working with multicolor images, time-lapse sequences, background handling and fluorescence quantification (hands on) and 4. Practical demonstration of wide-field fluorescence image acquisition using both an upright microscope and an inverted, automated microscope as well as spinning disk confocal microscopy (mix of demonstration and hands on). There will be a mandatory home assignment.

Recommended knowledge for participation (if any): This is an introductory course, so no experience with fluorescence microscopy is necessary

Language: English

ECTS: 6,0

Head of course: Associate Professor Lene N. Nejsum

Instructors: Mikkel Roland Holst, Frédéric H. Login, Didde Haslund

Number of participants: 27

Dates and times: 20-22 August and 27-29 August 2018 from 8:30 to 17:30

Place: Building: 1240-121 and adjacent laboratory facility, Wilhelm Meyers Allé 4, 8000 Aarhus C

Course fee: DKK 7200

Participation in the course is without cost for:

• PhD students, Research Year students and Research Honours Programme students from Aarhus University
• PhD students from University of Helsinki
• PhD students from other institutions in the open market agreement for PhD courses

Application deadline: 23-7-2018

Application: https://service.health.au.dk/modules/Course/mypage/coursecalendar.