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Advanced electron microscopy for cell biology
June 12 - June 22(Deadline: March 30)
Modern EM allows structure-function analysis by combining multidisciplinary approaches with physiological, genetic and biochemical data. Current advances in EM are wide ranging and focus on improving resolution, efficient 3D imaging, molecular mapping, correlative imaging and quantitation. Together they combine to make this approach amongst the most popular in biology. This EMBO Practical Course will provide the students with competence in a wide range of techniques, and then to learn how to integrate these effectively with other approaches in cell biology. Moreover, this EMBO Practical Course offers the opportunity to acquire a portfolio of cutting edge EM methods, allowing the participants to integrate EM tools and readouts into the workflow of their own cell biological research projects.
- State of the art fixation including cryo-methods
- Correlative light and electron microscopy
- Molecular mapping (immuno-EM)
- Three-dimensional EM imaging using tomography and serial imaging
- Advanced quantitative image analysis (stereology, nanomorphomics)
You will gain an intensive overview of the most advanced methods in electron microscopy for cell biology. We will provide training in project/experimental design, often adapted to each of the students projects. Moreover, the course has been running for over 30 years and has contributed to the creation of a wide community of electron microscopists (both students and teachers) all over the world.